Biomedical Engineering Reference
In-Depth Information
b
(a)
d
c
Green: fluorescence
Blue: SHG
200 μm
(b)
(c)
30 μm
30 μm
(d)
(e)
Segmentation
Bifurcation
30 μm
20 μm
FIgurE 12.5
(
See color insert.
) (a) Large-area, multiphoton autofluorescence (green) and backward SHG (blue)
images of the
ex vivo
human cornea that have been infected with
Alternaria
sp. (b), (c), and (d) represent the magni-
fied areas b, c, and d in (a). Parallel distributed fluorescence in the background and residual SHG-generating col-
lagen remnants were found in (b) and (c). Possible fungal hyphae with characteristic morphology of bifurcation and
segmentation (white arrows) can be visualized both in the large-scale (a) and detailed image (d). (e) Multiphoton
autofluorescence image of isolated
Alternaria
sp. (Adapted from Tan, H. Y. et al. 2007.
J Biomed Opt
12:024013.)
