Biomedical Engineering Reference
In-Depth Information
III
Applications of
SHG
11
Second-Harmonic generation Imaging of Self-Assembled
Collagen gels Christopher B. Raub, Bruce J. Tromberg, and Steven C. George.............. 245
Introduction • Background • SHG Imaging of Acellular Collagen Gels • SHG Imaging
of Cellularized Collagen Gels • Conclusions • Acknowledgments • References
12
SHg and Multiphoton Fluorescence Imaging of the Eye Chiu-Mei Hsueh,
Po-Sheng Hu, Wen Lo, Shean-Jen Chen, Hsin-Yuan Tan, and Chen-Yuan Dong ............. 271
Introduction • Principles of Second Harmonic Generation Microscopy • Visualization of
Normal Corneal and Surrounding Tissues • Imaging and Characterization of Pathological
Corneas • Future in Clinical Diagnosis • References
13
Multiphoton Excitation Imaging of the Arterial Vascular Bed Edward B.
Neufeld, Bertrand M. Lucotte, and Robert S. Balaban........................................................ 289
Introduction • Methodology and Preparations • Arterial Sample
Preparations • Investigation of Macromolecular Structure • Investigations of Vessel
Mechanics • Investigations of LDL Interactions with the Vascular Wall • Investigations
of the Diseased Vascular Wall • Summary and Future Directions • References
14
Combined SHg/THg Imaging Szu-Yu Chen and Chi-Kuang Sun ................................ 309
Excitation Laser Sources for SHG/THG Microscopy • Cr:F-Based SHG/THG
Microscopy • Biomedical Applications • Conclusion • References
15
SHg Imaging of Collagen and Application to Fibrosis Quantization
Marie-Claire Schanne-Klein .................................................................................................. 349
Introduction • Physical Origin of SHG Response from Collagen and Specificity to Fibrillar
Collagens • Second-Harmonic Imaging of Tissue Fibrosis • Fibrosis Quantitative Scoring
Using SHG Microscopy • Discussion: Advantages and Limitations of SHG Microscopy for
Fibrosis Scoring • Conclusion • Acknowledgments • References
16
Quantitative Approaches for Studying the role of Collagen in Breast Cancer
Invasion and Progression Caroline A. Schneider, Carolyn A. Pehlke, Karissa
Tilbury, Ruth Sullivan, Kevin W. Eliceiri, and Patricia J. Keely ......................................... 373
Collagen and Breast Cancer • Imaging Methods • Quantifying SHG Data • References
17
SHg in Tumors: Scattering and Polarization Seth W. Perry, Xiaoxing Han, and
Edward B. Brown .................................................................................................................... 391
Scattering Properties of SHG in Tumors • Polarization of SHG in Tumors • Summary
and Conclusions • Acknowledgments • Appendix: Theory of Polarization
Analysis • References
243
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