Biomedical Engineering Reference
In-Depth Information
I
Principles of
SHG Imaging
1
Theoretical Framework for SHg Microscopy: A Matrix-Based
Approach Christopher M. Dettmar and Garth J. Simpson ................................................... 3
Physical Overview • Mathematical Formalism • Special Considerations • Extension
beyond the Thin Sample Limit • Summary • Acknowledgments • References
2
How to Build an SHg Apparatus Riccardo Cicchi, Leonardo Sacconi, Francesco
Vanzi, and Francesco S. Pavone............................................................................................... 21
Mechanical System • Laser Scanning System • The Detection System • Optical Scheme
of a Typical SHG Microscope • References
3
THg Microscopy of Cells and Tissues: Contrast Mechanisms and
Applications Nicolas Olivier, Delphine Débarre, and Emmanuel Beaurepaire ................ 51
Introduction • THG Microscopy of Isotropic Media • THG Microscopy of Anisotropic
Media • Conclusion • Acknowledgments • Appendix • References
4
SHg Microscopy and Its Comparison with THg, CArS, and Multiphoton
Excited Fluorescence Imaging Xiyi Chen and Paul J. Campagnola ................................. 81
Introduction • Physical Origin and Contrast Mechanism of SHG and the Other
NLO Microscopies • Instrumentation of NLO Microscopes • Representative
Biological Applications of SHG Imaging and Its Comparison with Other NLO
Techniques • Summary • References
1
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