Biomedical Engineering Reference
In-Depth Information
I
Principles of
SHG Imaging
1
Theoretical Framework for SHg Microscopy: A Matrix-Based
Approach
Christopher M. Dettmar and Garth J. Simpson ...................................................
3
Physical Overview • Mathematical Formalism • Special Considerations • Extension
beyond the Thin Sample Limit • Summary • Acknowledgments • References
2
How to Build an SHg Apparatus
Riccardo Cicchi, Leonardo Sacconi, Francesco
Vanzi, and Francesco S. Pavone...............................................................................................
21
Mechanical System • Laser Scanning System • The Detection System • Optical Scheme
of a Typical SHG Microscope • References
3
THg Microscopy of Cells and Tissues: Contrast Mechanisms and
Applications
Nicolas Olivier, Delphine Débarre, and Emmanuel Beaurepaire ................
51
Introduction • THG Microscopy of Isotropic Media • THG Microscopy of Anisotropic
Media • Conclusion • Acknowledgments • Appendix • References
4
SHg Microscopy and Its Comparison with THg, CArS, and Multiphoton
Excited Fluorescence Imaging
Xiyi Chen and Paul J. Campagnola .................................
81
Introduction • Physical Origin and Contrast Mechanism of SHG and the Other
NLO Microscopies • Instrumentation of NLO Microscopes • Representative
Biological Applications of SHG Imaging and Its Comparison with Other NLO
Techniques • Summary • References
1