Biomedical Engineering Reference
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0 Deg.
45 Deg.
90 Deg.
5 μm
45 μm
5 µm depth
45 µm depth
1.1
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0
45
90
135
180
Angle with input polarization (deg.)
FIgurE 8.13 Angular dependence of the forward SHG intensity from control tendon at depths of 5 and 45 μm.
The representative optical sections at several polarization angles are shown in the top panels. The representative
error bars (standard error) are given for one laser polarization for each depth. (Adapted from Nadiarnykh, O. and
P. J. Campagnola. 2009. Opt. Express 17:5794-5806.)
1.0
Forward control
Forward cleared
0.8
0.6
0.4
0.2
0.0
-0.2
0
20
40
60
80
100
120
Depth (microns)
FIgurE 8.14 Forward SHG anisotropy β measured for forward SHG from control (black squares) and cleared
(gray squares) tendon. (Adapted from Nadiarnykh, O. and P. J. Campagnola. 2009. Opt. Express 17:5794-5806.)
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