Biomedical Engineering Reference
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(a)
(b)
(c)
CA1
CA3
DG
P18
(d)
(e)
(f )
P34
(g)
(h)
(i)
100 μm
100 μm
P369
0.5
(j)
160
140
0.4
120
0.3
100
80
0.2
60
40
0.1
20
0
0
0
100
200
Age (days)
300
400
FIgurE 7.6 Mapping the age-dependent distribution of polarized microtubules in native brain tissues. (a through
c) In an acute hippocampal brain slice from a 13-day-old mouse, SHG signals were observed in the axons of mossy
fibers, but not in the neuropil of the CA1 stratum radiatum region that primarily consisted of apical dendrites of
pyramidal neurons. (d through f, g through i) Same as (a) through (c) but for 34-day- and 369-day-old mice, where
SHG signals appeared in the CA1 stratum radiatum in an age-dependent manner, demonstrating that mature apical
dendrites contain polarized microtubule bundles. (j) Quantification of the length and density of microtubule bundles
in the CA1 stratum radiatum as a function of age. (Reprinted from Kwan, A. C. et al., 2008. Polarized microtubule
arrays in apical dendrites and axons. Proceedings of the National Academy of Sciences of the United States of America,
105, 11370-11375. Copyright 2008, with permission from the National Academy of Sciences, USA.)
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