Biomedical Engineering Reference
In-Depth Information
FIgurE 7.1
SHG imaging of mitotic spindles. (a) The first cell division of a fertilized sea urchin egg. (Reprinted
from
Methods
, 29, Mohler, W., Millard, A. C., and Campagnola, P. J. Second harmonic generation imaging of
endogenous structural proteins. 97-109. Copyright 2003, with permission from Elsevier.) (b) Multiple dividing sis-
ter cells in a zebra fish embryo. (Reprinted from Olivier, N. et al., 2010. Cell lineage reconstruction of early zebrafish
embryos using label-free nonlinear microscopy.
Science
, 329, 967-971. Copyright 2010, with permission from the
American Association for the Advancement of Science.) (c) An
in vitro
, cultured mouse oocyte. The arrowhead
indicates the zona pellucida, a boundary region that also generated SHG signal. (Reprinted from Hsieh, C. S. et al.,
2008a. Higher harmonic generation microscopy of
in vitro
cultured mammal oocytes and embryos.
Optics Express
,
16, 11574-11588. Copyright 2008, with permission from the Optical Society of America.) (d) Dividing rat baso-
philic leukemia cells in culture. The light gray arrow indicates the mitotic spindles and dark gray arrow indicates
interphase microtubule ensembles. (Reprinted from Dombeck, D. A. et al., 2003. Uniform polarity microtubule
assemblies imaged in native brain tissue by second-harmonic generation microscopy.
Proceedings of the National
Academy of Sciences of the United States of America
, 100, 7081-7086. Copyright 2003, with permission from the
National Academy of Sciences, USA.) Scale bar = (a) 45 μm, (b) 20 μm, (c) 60 μm, and (d) 10 μm.
basophilic leukemia cells (Dombeck et al., 2003). In the brain, SHG from microtubule bundles within
axons, nascent neurites, and mature apical dendrites have been reported in rat primary neuronal culture
(Dombeck et al., 2003, Kwan et al., 2008, Psilodimitrakopoulos et al., 2009), mouse primary neuro-
nal culture (Stoothoff et al., 2008),
Aplysia
neuronal culture (Mertz, 2004), rat acute hippocampal slice
(Dombeck et al., 2003), and mouse acute hippocampal and neocortical slices (Kwan et al., 2008, 2009,
