Biomedical Imaging Using SHG - Second Harmonic Generation Imaging - page 101

II

Biomedical
Imaging Using
SHG

5 Molecular Structure and Order with Second-Harmonic generation

Microscopy Francesco Vanzi, Leonardo Sacconi, Riccardo Cicchi, Chiara
Stringari, and Francesco S. Pavone ....................................................................................... 103
Introduction • From Hyper Rayleigh Scattering to SHG • SHG
Polarization Anisotropy • Membrane SHG Imaging • Endogenous SHG
Imaging • Source of the Endogenous SHG • Probing Protein Structural
Conformation • Conclusions • References

6 3D SHg Imaging and Analysis of Fibrillar Collagen Organization Paul J.

Campagnola............................................................................................................................. 125
Introduction • Methods • SHG Phasematching in Tissues • Results of 3D Imaging and
Analysis • Discussion • References

7 Second-Harmonic generation Imaging of Microtubules Alex C. Kwan..................... 151

Why Study Microtubules with SHG Imaging? • Application 1: Mapping the Distribution
of Polarized Microtubule Bundles in Native Brain Tissues • Application 2: Endogenous
Time Stamp and Marker for Cell Division for Whole-Embryo Developmental
Studies • Technical Notes for SHG Imaging of Microtubules • Summary • References

8 SHg and Optical Clearing Oleg Nadiarnykh and Paul J. Campagnola ......................... 169

Introduction • Physical Background • Monte Carlo Simulations • Mechanism of
Optical Clearing in Muscle • Optical Clearing in Collagenous Tissues • Optical Clearing
in Skin • Optical Clearing in Kidney Tissue • Alternative Investigation of Optical
Clearing: Artificial Environment • Retention of SHG Polarization Signatures through
Optical Clearing • Final Remarks • References

9 Holographic SHg Imaging Etienne Shaffer and Christian Depeursinge ....................... 191

Introduction • Principle of Holography • Recording of Digital SHG
Holograms • Reconstruction of Digital SHG Holograms • Image Contrasts
in Digital Holographic SHG Imaging • Selected Applications • Concluding
Remarks • Acknowledgments • References

10 Imaging Membrane Potential with SHg Mutsuo Nuriya and Rafael Yuste ................. 229

Introduction: Membrane Potential Measurements with Imaging • SHG:
Theoretical Background • SHG Voltage Chromophores • Experimental
Setup • Applications • Conclusions and Perspectives • References

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