Biomedical Engineering Reference
In-Depth Information
a
Time (min)
b
Time (min)
Fig. 6. Investigation of kinetic properties of immobilized invertase by flow microcalorimetry
in the circulation mode. Initial sucrose concentration 51 mM, invertase immobilization by
biospecific binding on concanavalin A-bead cellulose was prepared by binding on concana-
valin A linked to chlorotriazine-activated cellulose. a Raw experimental thermometric data;
b data after conversion by the procedure indicated in Fig. 4.Concentrations were determined
spectrophotometrically ( open symbols ) and by transformation of thermometric data explained
in Section 5 ( closed symbols ) [32]
5.1.2
Reaction Systems Without Limitation by Particle Diffusion
In many cases the influence of particle mass transfer on the reaction rate can
be neglected (value of Thiele modulus approaches zero or the enzyme is immo-
bilized exclusively on the particle surface). Then, value of the effectiveness fac-
tor is unity and the solution of Eqs.(1)-(3) is substantially simpler.
Search WWH ::




Custom Search