Biomedical Engineering Reference
In-Depth Information
mol min -1 )
Reaction rate (
m
Fig. 5. Correlation between heat response and reaction rate of cephalosporin C transforma-
tion by immobilized D -amino acid oxidase of Trigonopsis variabilis . Enzyme immobilization
techniques: entrapment in polyacrylamide gel ( ),cells cross-linked with glutaraldehyde ( ),
cells entrapped in polyacrylamide gel (
) [28]
the slope calculated by linear regression and its value was
a = 4.44 K
(µmol
min -1 ) -1 [28].
Since each calibration point is performed in a newly packed column,there is
important data dispersion around the calibration line. In addition, the entire
calibration procedure is rather time consuming. Therefore, another approach
was developed, that provides more accurate results in a considerably shorter
time.This recently published approach [32] employs a slightly modified version
of the same FMC equipment. Instead of a flow-through arrangement enabling
the measurement in steady state, the measurement is performed in a system
with continuous circulation,as shown in Fig. 1.
The method was confirmed experimentally with sucrose hydrolysis catalyzed
by invertase,that had been immobilized by biospecific binding on concanavalin
A-bead cellulose. Figure 6B shows good agreement between the substrate con-
centration determined by the conversion of thermometric signals of Fig. 6A and
those obtained by spectrophotometric analysis.From the data shown in Fig. 6B,
the initial rate was determined to be v 0 = 0.775 mM min -1 .Introducing this value
into Eq. (23),v obs was calculated and the value of the transformation parameter
a
was determined from Eq. (24).
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