Biomedical Engineering Reference
In-Depth Information
Table 3. TELISA systems
Analyte
Immobilized
Concentration range Reference
compound
[
m
g/ml]
albumin
immobilized antibody
> 10 -10 mmol/l
Mattiasson et al.1977
gentamicin
and enzyme-labeled
> 0.1
Danielsson et al.1981a
insulin
antigen
0.1-50
Birnbaum et al.1986;
Mecklenburg et al.1993
human
IgG
protein
A50-500
Lammers
(polyclonal)
protein G
50-600
Lammers 1996
rabbit
IgG
protein
A10-150
Lammers
(polyclonal)
protein G
20-1000
Lammers 1996
anti-rabbit-IgG
10-200
Grau 1993
mouse
IgG
protein
A100-2000
Brandes
(monoclonal) anti-mouse-IgG
5-200
Lammers 1996
rt-PAanti-rt-PA
-IgG
5-50
Lammers
1996
Table 4. Enzyme activities determined with an ET
Enzyme
Mixed with
Concentration
Reference
range [units/ml]
acetylcholine esterase no mixing;
0.2-4
Lammers 1996
immobilized choline
oxidase + catalase
arginase
no mixing;
0.39-100
Lammers 1996
immobilized urease
catalase
H 2 O 2
20-60
Sauerbrei 1988
b
-galactosidase
lactose
0.5-4
Hundeck 1988
glucose oxidase
no mixing;
0.29-80
Lammers 1996
immobilized catalase
glucose-6-phosphate glucose-6-phosphate
0.2-3.0
Danielsson et al.1981b
hydrogenase
+ NAD +
hexokinase
glucose + ATP
0.1-2.5
Danielsson et al.1981b
lactate dehydrogenase pyruvate + NADH
0-20
Flygare et al.1990
peroxidase
H 2 O 2
0.2-4
Hundeck 1988
urease
urea
0.1-100
Danielsson 1979
via urea release.Figure 4 shows schematically a set-up for arginase monitoring
during its chromatographic FPLC purification.The computer-controlled system
facilitates a fast fraction-wise allocation of arginase activities.
Flygare et al. (1990) demonstrated ET monitoring of an affinity-adsorption
procedure.Lactate dehydrogenase (LDH) was recovered from a crude solution
by affinity binding to a N 6 -(6-aminohexyl)-AMP-sepharose gel.The LDH activi-
ty signal from the ET was used in a PID controller to regulate the addition of
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