Thermal Biosensors in Biotechnology - Advances in Biochemical Engineering Biotechnology - page 34

Thermal Biosensors in Biotechnology

Frank Lammers · Thomas Scheper
Institute for Technical Chemistry,Callinstraße 3,D-30167 Hannover,Germany
E-mail: [e-mail]
E-mail: [e-mail]

The application of enzyme thermistor devices for the continuous monitoring of enzymatic
processes is described. Different hardware concepts are presented and discussed, practical
results are also given. These devices were used to analyze the enantiomeric excess in bio-
transformation processes and for thermal immunoanalysis.In addition,the biosensors were
applied for the monitoring and control of an L -ornithine producing process and for the appli-
cation in hemodialysis monitoring.A review section discusses the use of thermal biosensors
for monitoring biotechnological processes in general.
Keywords: Biosensors,Enzyme thermistor,Process monitoring,Enzyme Technology,Biotrans-
formation.

1I tr ti n . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

2 Thermal Biosensors:Principles and State of the Art . . . . . . . . . 37

3 Applications of Enzyme Thermistors - A Review . . . . . . . . . . . 39
3.1 Clinical Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
3.2 Immunoanalysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
3.3 Determination ofEnzyme Activities . . . . . . . . . . . . . . . . . . 42
3.4 Process Monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
3.5 Environmental Analysis . . . . . . . . . . . . . . . . . . . . . . . . . 48
3.6 Enzymatic Amplification . . . . . . . . . . . . . . . . . . . . . . . . . 49

4 New Fields for Enzyme Thermistors . . . . . . . . . . . . . . . . . . 50
4.1 Enantiomeric Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . 50
4.2 Aminoacid Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
4.3 Medical Monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
4.4 Kinetic Characterization of Immobilized Biocatalysts . . . . . . . . . 56
4.5 Monitoring ofEnzyme Catalyzed Syntheses . . . . . . . . . . . . . . 56
4.6 Monitoring in Food Technology . . . . . . . . . . . . . . . . . . . . . 59

5 New Hardware Concepts . . . . . . . . . . . . . . . . . . . . . . . . . 60
5.1 High Resolution Thin-Film Thermistors . . . . . . . . . . . . . . . . 60
5.2 Miniaturized Enzyme Thermistors . . . . . . . . . . . . . . . . . . . 61
5.3 Integrated Thermopiles . . . . . . . . . . . . . . . . . . . . . . . . . . 62