Bioaffinity Based Immobilization of Enzymes - Advances in Biochemical Engineering Biotechnology - page 213

Fig 2. Schematic representation of the bioaffinity layering of glycoenzyme on a concanavalin
A support
glucose and peroxide. An effective enzyme thermistor containing Con A
Sepharose and the glycoenzyme L -ascorbate oxidase was also described [129].
More recently Köneke et al. [130] described a fluoride sensitive field-effect
transistor (Si/SiO 2 /Si 3 N 4 /LaF 3 layers) based biosensor with Con A immobilized
on the basic membrane for the continuous measurement of glucose concen-
tration. The glycoenzymes glucose oxidase and peroxidase were bound on Con
A immobilized on the surface on the sensor. The enzymes could be readily
removed from the sensor surface with acetate solution pH 1.0 and the sensor
reloaded with fresh enzyme. The biosensor could be successfully integrated in
a flow-injection analysis system for continuous monitoring of glucose during
the cultivation of S.cerevisiae. We have also recently employed a cartridge con-
taining bioaffinity layered glucose oxidase on Con A-Sepharose support
connected to an oxygen electrode for the sensitive measurement of glucose
concentration during the fed-batch of cultivation of the S. cerevisiae on a syn-
thetic medium [116].