Bioaffinity Based Immobilization of Enzymes - Advances in Biochemical Engineering Biotechnology - page 200

4 Enzyme Immobilization Using Other Bioaffinity Supports . . . 218

4.1 Enzyme Immobilization on Immobilized Metal ion Supports . . 218

4.2 Immobilization of Chimeric Enzymes . . . . . . . . . . . . . . . 222

5 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 223

List of Abbreviations

BSA Bovine serum albumin

CBD Cellulose binding domain

Con A Concanavalin A

Glc Glucose

GlcNAc N -acetyl glucosamine

HRP Horse radish peroxidase

IDA Iminodiacetic acid

IgG Immunoglobulin gamma

IMA Immobilized metal affinity adsorption

Man Mannose

NTA Nitricotriacetic acid

1
Introduction

Developments in the areas of recombinant DNA technology,protein engineering
and more recently solvent engineering [1,2] have remarkably enhanced the
potential of enzymes in catalyzing the transformation of both water soluble and
water insoluble substances,for industrial and analytical applications. Most of
the enzyme applications necessitate homogeneous or at least reasonably pure
preparations that tend to be expensive. Recovery,reuse and stabilization of the
enzymes in the usually unphysiological or even hostile environments to which
they are exposed during operation therefore becomes obligatory in order to
make the transformations and analyses cost-effective. The now rather mature
enzyme immobilization technology offers a spectrum of strategies and a judi-
cious choice amongst these is likely to enhance the performance of any given
enzyme [3,4]. While interest in irreversible and covalent methods of immobi-
lization continues,bioaffinity based procedures are gaining remarkable atten-
tion especially for analytical applications [5,6]. Compared to other methods of
immobilization,those based on bioaffinity offer several distinct advantages.
These include:

1. Binding of the enzyme to affinity support may be very strong yet reversible
under specific conditions.
2. Immobilization process is usually simple,mild and necessitates no special
skills.