Biomedical Engineering Reference
In-Depth Information
a
b
Fig. 8.
a
Schematic of the set-up for simultaneous electrochemical and thermometric deter-
mination of analytes (for a detailed explanation, see text).
b
Optimization of catechol detec-
tion using hybrid (electrochemical and thermometric sensing) for oxygen concentration and
its effect on sensitivity of thermal detection [30]
3
Applications
3.1
General Applications
The metabolites determined using ET devices include alcohols, glucose, and
lactate using alcohol oxidase [22], glucose oxidase [23] and lactate oxidase re-
spectively. The detection limit for these compounds in pure solutions was in the
submicromolar region. The normal procedure was to co-immobilize the oxida-
ses with catalase in order to increase the total heat production, reduce the oxy-
gen consumption, and eliminate the hydrogen peroxide. Cellobiose was mea-
sured by using
-glucosidase in combination with glucose oxidase and catalase.
Determination of cholesterol and cholesterol esters was accomplished using
cholesterol oxidase and cholesterol esterase, and of triglycerides by using lipase.
b
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