Biomedical Engineering Reference
In-Depth Information
Regardless of the method, exons with highly repetitive and high GC regions are not
well enriched and will require Sanger sequencing for completeness and confi rma-
tory testing (Valencia et al. 2012 ).
The currently popular vision that a clinical laboratory with a single benchtop
machine could replace a large sequencing center can only be realized with increases
in the productivity of computers and bioinformaticians even more dramatic than
that expected for sequencers. It appears that for an individual $1,000 genome
sequences to be truly useful, fundamental advances in computation and bioinfor-
matics will be essential for data handling, storage, and interpretation. The obvious
importance of computational analysis of sequence data has led to a greater overall
appreciation of the role of theory in biology. A relationship between theory and
experiment, not unlike that found in the twentieth century physics, seems to be
taking shape.
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