Biomedical Engineering Reference
In-Depth Information
Figure 44.3.
Phase-contrast microphotographs of bovine artery ECs cul-
tured on PIPAAm-1.4 (a) and PIPAAm-2.9 (b) at 37
◦
C for 1 day. Although
cells can adhere and harvest onto PIPAAm-1.4 at 37
◦
C, cells cannot attach
onto PIPAAm-2.9 at 37
◦
C. Reprinted with permission from Ref. 20. Copy-
right 2004American Chemical Society.
Insummary,thethicknessandtheamountofgraftedPIPAAmlay-
ershaveasignificantinfluenceonthesurfacepropertiesintermsof
temperature-responsive cell adhesion and detachment, and a thick-
ness of approximately 20 nm is a key factor for the PIPAAm-grafted
surface to achieve cell adhesion and detachment with tempera-
ture. The progressive dehydration of PIPAAm chains existing at the
vicinity of hydrophobic TCPS may be important to the cell adhesion
characteristics at 37
◦
C. In contrast, the graft polymer at the outer-
most interfaces of PIPAAm-2.9 should be more polar at 37
◦
C than
PIPAAm-1.4,which inhibitscells from adhering.
44.3 Temperature-Responsive Cell Culture Surfaces that
Enable A
nity Control
Specific interactions between biomolecules and their molecular
recognitionplayimportantrolesinbiologyandphysiology.Inpartic-
ular,interactionsbetweencellreceptorsandbioactiveECMproteins
arenecessarytomaintainmultiplecellfunctions.Appropriatechem-
ical methodologies for biomaterial designs that regulate the bioac-
tivities of proteins and cells are becoming increasingly popular.
22
,
23
Particularly, the control of specific interactions between bioac-
tive molecules has been used to study, understand, and con-
trol molecular recognition-mediating biological processes.
24
,
25
The
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