Biomedical Engineering Reference
In-Depth Information
Figure 44.3. Phase-contrast microphotographs of bovine artery ECs cul-
tured on PIPAAm-1.4 (a) and PIPAAm-2.9 (b) at 37 C for 1 day. Although
cells can adhere and harvest onto PIPAAm-1.4 at 37 C, cells cannot attach
onto PIPAAm-2.9 at 37 C. Reprinted with permission from Ref. 20. Copy-
right 2004American Chemical Society.
Insummary,thethicknessandtheamountofgraftedPIPAAmlay-
ershaveasignificantinfluenceonthesurfacepropertiesintermsof
temperature-responsive cell adhesion and detachment, and a thick-
ness of approximately 20 nm is a key factor for the PIPAAm-grafted
surface to achieve cell adhesion and detachment with tempera-
ture. The progressive dehydration of PIPAAm chains existing at the
vicinity of hydrophobic TCPS may be important to the cell adhesion
characteristics at 37 C. In contrast, the graft polymer at the outer-
most interfaces of PIPAAm-2.9 should be more polar at 37 C than
PIPAAm-1.4,which inhibitscells from adhering.
44.3 Temperature-Responsive Cell Culture Surfaces that
Enable A nity Control
Specific interactions between biomolecules and their molecular
recognitionplayimportantrolesinbiologyandphysiology.Inpartic-
ular,interactionsbetweencellreceptorsandbioactiveECMproteins
arenecessarytomaintainmultiplecellfunctions.Appropriatechem-
ical methodologies for biomaterial designs that regulate the bioac-
tivities of proteins and cells are becoming increasingly popular. 22 , 23
Particularly, the control of specific interactions between bioac-
tive molecules has been used to study, understand, and con-
trol molecular recognition-mediating biological processes. 24 , 25 The
 
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