Biomedical Engineering Reference
In-Depth Information
Chapter 44
TEMPERATURE-RESPONSIVE CULTURE
SURFACES FOR REGENERATIVE MEDICINE
Yoshikazu Kumashiro, Yoshikatsu Akiyama, Masayuki Yamato, *
and Teruo Okano **
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical
University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan
*myamato@abmes.twmu.ac.jp; **tokano@abmes.twmu.ac.jp
Cell-based sheet engineering using temperature-responsive cell
culture surfaces was developed as a novel strategy for regener-
ative medicine to overcome the limits of conventional methods
such as single-cell suspension injection and biodegradable poly-
mer scaffolds. Here, we summarize the basic mechanisms of cell
attachment and detachment and cell patterning on temperature-
responsive cell culture surfaces to allow for rapid cell sheet
fabrication and recovery. In addition, we show in vivo transplan-
tations of cell sheets, with possible applications such as corneal
reconstruction, periodontal ligament transplantation, esophageal
reconstruction, and the sealing of lung air leaks.
44.1 Introduction
Tissue engineering utilizing biodegradable scaffolds has been pur-
sued for its utility in the area of regenerative medicine. 1 , 2 Although
early clinical treatment of cartilage has been reported, 3 clinical
 
 
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