Biomedical Engineering Reference
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Figure 43.18.
FDA/PI live/dead staining of the cellular scaffolds. The
bioreactor culture led to complete cellular confluence in the scaffolds at
week 2, while the static culture failed to do so. At week 4, the bioreactor
culture maintained the high viability of cellular scaffolds, while the static
cultureresultedinlargecellularnecrosis(livecellswerestainedingreenby
FDA; dead cells were stained in red by PI).See also ColorInsert.
immunodeficient mice (3.2x, p
<
0.001) compared with static-
cultured scaffolds.
73
The use of the biaxial rotating bioreactor here
allowed the maintenance of cellular viability beyond the limits of
conventional diffusion, with increased proliferation and osteogenic
differentiationboth
in vitro
and
in vivo
,suggestingitsutilityforBTE
applications.
On the basis of our expertise in scaffold fabrication, human fetal
stem cell research, and bioreactor design, we conceptualized a BTE
strategy by seeding hfMSCs onto a PCL-TCP scaffold, culturing and
maturing cellular scaffolds into TE bone grafts. A critical-sized rat
femoral defect model was selected to testify our concept and eval-
uate the effectiveness of our TE bone grafts for the defect healing.
Results showed that the implantation of this TE bone graft can pro-
mote new bone formation and neovascularization and successfully
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