Biomedical Engineering Reference
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Figure 43.12.
A The fibrin/human BM stem cells composite scaffold
in
vitro
12 days after induction of differentiation to judgment of differen-
tiation ability to osteoblasts. Osteogenic differentiation was seen in test
tube(A). Osteogenic differentiation in a section of fibrin/human BM stem
cells composite scaffold was observed. (B-1) Fibrin/human BM stem cells
composite scaffold in a normal growth medium. (B-2) Calcified matrix
(black) is secreted by the human BM stem cells/fibrin composite. (con,
control; test, experiment.) See also Color Insert.
weeks reduced the fibrin glue scaffold size, which was visible in all
groups. The defect region was not filled with newly formed bone
(Figs. 43.15a-c). That is, osteoid formation and woven bone struc-
tures were not seen. There were no significant differences among
the groups. Also, a foreign-body reaction could be observed at the
fibrin glue scaffold-host bone interface. But the foreign-body reac-
tion was reduced gradually during 12 weeks.
22
The activity of BM
stem cells seemed to beweakbecause of rejection.
To conclude, our study could not demonstrate the potential of
bone regeneration in a fibrin glue scaffold
in vivo
. Toward a clini-
cal application, further investigations have to be done using human
BM stem cells, such as using immunosuppression agents. The per-
formance of the composite constructs has to be assessed in a rele-
vantimplantationassociatedwithaspecificnutrientandmechanical
situation.
23
,
24
Thepresentapproachwillcometoguidemoremature
research forTE using BM stem cells andscaffolds.
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