EFFECT OF SCAFFOLDS WITH BONE GROWTH FACTORS ON NEW BONE FORMATION - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 875

Figure 43.5. At 3 weeks and even 6 weeks, the percentage of BMD in
group I increased significantly compared with control groups II and III.
Whitebox,groupI;blackbox,groupII;hatchedbox,groupIII.SeealsoColor
Insert.
was higher than the densities of the two control groups (group II,
6.9%; group III, 7.1%). At week 6, the percentage BMD of group I
(58.4%) was significantly higher than that of group II (10.2%) and
of group III (10.4%) (P < 0.01). However, there were no signifi-
cant differences between the two control groups—groups II and III
(Fig. 43.5).
Fromthehematoxylinandeosin(H&E)histologicalstudiesinthe
two control groups, the new bone formation around the cut end of
thebonehadabundantcollagenousfibroustissuewithsomeportion
ofbonytrabeculaeinthedistractiongap.However,withoutbridging
between the two cut ends, the bone formation was incomplete in all
control animals (Fig. 43.6). In all rabbits from group I with calcium
sulfate, active new bone formation in the distracted area was noted.
A similar trabecular pattern of bridging was also noted at the origi-
nalcutboneends,butthisbridgingwasthin.Theremainingcalcium
sulfate was nearly invisible. This study has shown that the implan-
tationofcalciumsulfatepromotesbonematurationinthecontextof
distraction osteogenesis.