Biomedical Engineering Reference
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Figure 42.9.
Detection of hydrogen peroxide using chemiluminescent
micelles.
The chemiluminescent micelles have a mean size of 50 nm and
possess a stealth PEG corona to enhance their circulation in the
bloodstream.Theywereabletodetecthydrogenperoxideatconcen-
trations as low as 50 nM (Fig. 42.9). The significant enhancement of
sensitivity is due mainly to the small size, which provides a larger
surface area and enhances the diffusion of hydrogen peroxide into
the micelles. The small size of chemiluminescent micelles should
make them suitable for imaging extracellular hydrogen peroxide
produced in inflammatory tissues as micelles with a mean size of
50 nmcan extravasate into inflammatory tissues.
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42.4 Fluorescent Probes for Other ROS
Superoxide is known to be first generated in the respiratory chains
and dismutes spontaneously to hydrogen peroxide.
8
,
29
-
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The pro-
duction and reaction of superoxide as a precursor to other ROS
are of considerable importance to studies of cellular metabolism
and pathogenesis of diverse cytotoxic phenomena. Therefore, there
has been great interest to detect superoxide to elucidate the
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