Biomedical Engineering Reference
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Figure 42.4.
Fluorescent probes with diphenylphosphine moiety of
hydrogen peroxide.
levelofhydrogenperoxidewithinthemitochondriaofvariousmam-
malian cells, as well as hydrogen peroxide elevation induced by an
oxidant stress model of Parkinson'sdisease.
Another approach for the selective detection of hydrogen per-
oxide is based on the concept of photoinduced electron transfer
(Fig. 42.4), in which 7-Hydroxy-2-oxy-
N
-(2-(diphenylphosphino)
ethyl)-2H-choromene-3-carboxamide was rationally designed to
consist of diphenylphosphine moiety and 7-hydroxycoumarin
moiety.
25
With the diphenylphosphin moiety, 7-hydrocoumain had
limited fluorescence. It produced increased fluorescence upon oxi-
dation by hydrogen peroxide. However, it showed undesirably low
reactivity toward hydrogen peroxide, fluorescence appearing over
tens ofminutes at 50
μ
M ofhydrogen peroxide.
42.3 Luminescent Probes for Imaging
of Hydrogen Peroxide
Peroxalate chemiluminescence is the most effective and sensi-
tive chemiluminescence and is based on the reaction between an
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