Biomedical Engineering Reference
In-Depth Information
MSC survivaland proliferation. Successful culture ofMSCs inan ani-
malserum-freemediumsupplementedwithgrowthfactorshasbeen
reported by Ng and Gronthos.
80
These MSCs were able to propa-
gate, at least to limited passages. These culture conditions may also
be combined with other FBS substitutes, such as platelet lysates, as
recently described by Schallmoser.
1
The ultimate goal for the
ex vivo
expansion of MSCs is to
produce safe and su
cient MSCs for therapeutic application. In
order to achieve this, a combination of growth factors that exert a
stimulatory effect on MSC proliferation when used in combination
with other factors, such as autologous serum, needs to be evalu-
ated in MSCs derived from large numbers of donors. On the basis
of the advancements made thus far, it seems likely that the ability to
expand MSCs under therapeutically safe and effective culture condi-
tionswillbein our hands in thenear future.
Acknowledgments
Research described in this chapter was supported by a grant of the
Korea Healthcare technology R&D Project, Ministry for Health, Wel-
fare & Family Affair, Republic of Korea (A080840 and A091224). I
would like to thank Dr. So Young Chun, Dr. Young Ae Choi, and Ms.
Jiwon Lim for preparation of the early drafts and Ms. Hye-Jung Noh
for technical assistance.
References
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