Biomedical Engineering Reference
In-Depth Information
There were no signs of apoptosis, inflammation, or necrosis in
any site, which was expected because the cultured cells were autol-
ogous.
The results of our morphologic and morphometric analyses sug-
gest that cross-linked HA and fibroblasts lead to additional synthe-
sisofextracellularcomponents(i.e.,fibersandgroundsubstance)in
connective tissue. This supports Yoon's results,
18
-
24
which showed
thatcross-linkedHAcombinedwithculturedhumanfibroblastspro-
vides a shorter appearance of effect and a longer-lasting effect com-
pared withfibroblastsalone.
Thestudydemonstratesthatculturedhumangingivalfibroblasts
combinedwithHAcanbeasuitable,biocompatible,andlong-lasting
materialandshouldberegardedasanewmethodindermalrenova-
tion in dentistry.
35.5 Conclusion
Tissue engineering has opened a new era for more permanent filler
materialsbyenablingcombinationsofcellelementswithbiodegrad-
able polymer scaffolds. In our study, cross-linked HA was used as a
biodegradable polymer scaffold for cultured human gingival fibrob-
lasts, which are considered “biomaterials that heal.” Living autol-
ogous gingival fibroblasts with HA have the potential to provide
higher aesthetic results and satisfaction to patients. It opens a new
window in the field of oral surgery.
References
1. M. Ueda,
Biochem. Soc.
,
11
(2007).
2. F. D. Sarosh, D. M. Carl, and Hom-Lay wang,
J. Oral Implantol.
,
191
(2007).
3. M.A.Biondi and S. C.Brown,
Phys. Rev.
,
1700
(1949).
4. E.S.Yoon, S. K.Han, and W.K.Kim,
Ann. Plast. Surg.
,
51
,587 (2003).
5. J.B.A.Mitchell,
Phys. Rep.
,
215
(1990).
6. F. Duranti, G. Salti, B. Bovani, M. Calandra, and M. L. Rosati,
Dermatol.
Surg.
,
24
,1317 (1998).
Search WWH ::
Custom Search