Biomedical Engineering Reference
In-Depth Information
containing 0.1 μ g/mL ethidium bromide. The products were ana-
lyzed using the ChemiDoc XRS gel documentation system (Bio-Rad
Laboratories).
Before cell injection, contamination was checked for again. The
supernatant of each cell culture flask used was collected five days
before cell harvest and tested as described for prepared the tissue
culture medium.
Onthedayofinjection,5
μ
Lofthecellsuspensionthathadbeen
preparedforinjectionwasdiluted100-foldinnormalsalinesolution
and tested using the Endosafe PTS system (Charles River Laborato-
ries) for endotoxin before injection. Samples containing more than
1.0 EU/mL were considered positive.
35.2.6 Clinical Assessment of Aesthetic Improvement
Allsatisfactory assessmentswereperformed bythepatient, andthe
followinggradingscalewasusedat3,6,12,and36monthsafterthe
first injection:
4. Completely satisfied
3. Satisfied
2. No remarkable change observed
1. Notsatisfied
35.2.7 Skin Replica and Analysis
At the time of wrinkle induction and one week after the final injec-
tion of the gingival fibroblast and HA admixture, negative replicas
of the skin surface were taken by using a silicon-based impression
material, Flextime R (Heraeus Kulzes, New York). To obtain repli-
cas of the wrinkles from the same skin area, the skin was marked
using an oil-based marker pen. For ease of measurement, all repli-
cas were cut into 1 cm square pieces, and the back of each replica
was processed into a flat plane using the same impression material.
Light was directed at a 20-degree angle, and images were incor-
porated from replica using a charge-coupled device (CCD). The
image of the negative replica was observed using a wrinkle analy-
sis system skin visiometer SV 600 (Courage & Khazaka, Cologne,
 
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