PGA FIBER FOR SOFT TISSUE ENGINEERING - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 695

Figure 34.10. Histological structure and staining of engineered tissues
after subcutaneous implantation. After 12 weeks of implantation, tissue
engineeredby4weeksofinduction in vitro mainlyformedossifiedtissue(a,
solid arrow) with strong positive staining of Azan-Mallory (d). Blood ves-
sels (A, rectangle), fibrous tissue (a, dotted arrow), and positive staining
of type II collagen (b, arrow) were also observed in a part of some spec-
imens. No obvious positive staining of Safranine O was observed (c). The
cartilage engineered by 8-week induction in vitro mainly formed cartilage-
like tissue (e) with positive staining of type II collagen (f) and Safranine O
(g)afterimplantation.Nevertheless,positivestainingofAzan-Mallorycould
still be observed in the edge area of some specimens (h, arrow). For the
cartilage engineered by 12-week induction in vitro ,however,allthespec-
imens showed cartilage-like tissue (i, m) with strong, positive staining of
type II collagen (j, n) and Safranine O (k, o), as well as negative staining of
Azan-Mallory (l, p) after 12 (i-l) or 24 (m-p) weeks of implantation, and
neitherossifiedtissuenorfibroustissuewasobserved.Scalebar = 100 μ m.
(Reprinted by permission from Ref. 17). See also Color Insert.