NOVEL FABRICATION AND CHARACTERIZATION OF PORE-SIZE-GRADIENT SCAFFOLDS BY A CENTRIFUGATION TECHNIQUE - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 603

Figure 30.10. Number of cells adhered and grown in pore-size-gradient
chitosan scaffold sections; (a) chondrocytes, (b) osteoblasts, and (c) fibrob-
lasts. The cell numbers were determined from the MTT assay calibration
curve ( ∗ , average pore size; n = 3) (Ref.29).

Figure 30.11. Number of cells adhered and grown in pore-size-gradient
PCL scaffold sections; (a) chondrocytes, (b) osteoblasts, and (c) fibroblasts.
The cell numbers were determined from the MTT assay calibration curve
( ∗ , average pore size; n = 3)(Ref.28).

30.3.5 In vivo Tissue Interactions
To evaluate the pore size effect on in vivo tissue interactions, the
selected sections of the pore-size-gradient, cylindrical PCL scaffold
were implanted into the skull bone defects of a rabbit. For this,
healthy and skeletally mature male New Zealand white rabbits with
weight between 2.0 and 2.5 kg were selected and cared for accord-
ing to the Korean Food and Drug Administration guidelines for the
care and use of laboratory animals. The animals were housed in
individual cages. Surgery was performed under general anesthesia.
The anesthesia was induced by an intramuscular injection of keta-
mine hydrochloride (10 mg/kg, Ketala R , Yuhan Co., Korea) and 2%
xylazine hydrochloride (2 mg/kg, Rumpun R , Byely Co., Korea).
To reduce the perioperative infection risk, the rabbits received