NOVEL FABRICATION AND CHARACTERIZATION OF PORE-SIZE-GRADIENT SCAFFOLDS BY A CENTRIFUGATION TECHNIQUE - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 601

Figure 30.9. Photographs demonstrating the wettability of a cell cul-
ture medium into (a) alginate or chitosan, (b) PCL (no hydrophilized), and
(c) PCL (hydrophilized by Pluronic F127 addition) scaffold sections at the
position of 2.0 ∼ 2.5 cm (Ref.22).

into the 12-well polystyrene (PS) dishes (Corning, USA). Three dif-
ferent types of cells, fibroblasts (NIH/3T3 mouse embryo fibrob-
lasts, Korean Cell Line Bank, Korea), chondrocytes (immortalized
human costal chondrocyte cell line C-28/12, Beth Israel Deaconess
MedicalCenter,USA),andosteoblasts(MG-63humanosteosarcoma,
Korean Cell Line Bank), were used as model cells to estimate the
pore size effect on cell interactions. A cell suspension in the Roswell
Park Memorial Institute medium (RPMI, for fibroblasts) or Dul-
becco's Modified Eagle's Medium (DMEM, for chondrocytes and
osteoblasts) containing 10% fetal bovine serum (FBS, Gibco, USA),
0.1% gentamicin sulfate (Sigma, USA), and 1% penicillin G (Sigma)
was seeded onto the each scaffold section (cell density 1 10 6

cells/specimen). The cell suspensions could be easily wetted into

×