Biomedical Engineering Reference
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Figure 30.2.
Schematicdiagramsshowingthefabricationprocessofpore-
size-gradient, cylindrical scaffolds by the centrifugation method (Ref. 22).
30.2.2
Pore-Size-Gradient Alginate Scaffolds
To prepare alginate cylindrical scaffolds having a pore size gradi-
ent, 2wt% alginatesolution wasslowly dropped into a 2wt%CaCl
2
solution with vigorous agitation using a homogenizer (20,000
rpm; HG-3000, SMT, Japan) to obtain fibril-like cross-linked algi-
nate structures (Fig. 30.2). The prepared fibril-like alginates were
washedinexcesswatertoremoveresidualCaCl
2
.Thenthefibril-like
alginate-suspended aqueous solution was poured into a polypropy-
lene (PP) cylindrical mold (inner diameter 14 mm; height 7.5 cm),
and the mold was centrifuged (3,000 rpm) for five minutes. During
the centrifugation, the fibril-like alginates were accumulated in the
cylindrical mold with a range of pore size gradients by the gradual
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