Biomedical Engineering Reference
In-Depth Information
Figure 28.6. Optical microscopic photographs of a collagen mesh (a,
diameter = 15 mm) and a reinforced collagen sponge with collagen mesh
(c). Scanning electron microscopy of a collagen thread (b) and a rein-
forcedcollagenspongewithacollagenmesh(a).Originalmagnification:(b)
×
1500;(d)
×
35,scalebar
=
(b)10 μ m:(d)1,000 μ m.(Seo et al .,Biotechnol.
Bioprocess Eng. 1998)
Recently, Okochi et al . attempted to increase wound healing
through enhanced structural stability using collagen sponges. A col-
lagen sponge reinforced with PGA was developed, which increased
hair formation and growth when compared with a typical colla-
gen sponge (Fig. 28.6). Epidermal cysts and ectopic hairs formed
on the collagen sponge without PGA, and the volume of the sponge
decreasedasaresultofhumidityafterseedingofthecellsuspension.
This resulted in the pores of the collagen sponge shrinking, which
may have inhibited the movement of the transferred cells and led to
the formation of epidermal cysts forming at the grafted site and the
induction of cell leakage and loss.
However, the collagen sponge reinforced with PGA underwent
less shrinkage, thereby maintaining the size of the pore structures
so that the grafted cells could be retained. 77 Recently, a novel type
of reinforced scaffold that had high biocompatibility and strong
mechanicalpropertieswasdesigned.Thiscollagenspongewasrein-
forced with a collagen mesh using a combination of lyophilization
and cross-linking methods, which resulted in a structure composed
of collagen threads and the optimal materials for prevention of an
inflammatory reaction. 78
 
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