REINFORCED SCAFFOLD FOR TISSUE ENGINEERING - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 567

Figure 28.2. Gross appearance (a, b) and scanning electron microscopy
(c, d) of the silk scaffold (a, c) and reinforced collagen-hyaluronan scaffold
with silk thread (b, d). (Circles: Cross-section of silk thread). Scale bar = a,
b: 1,000 μ m; c, d 100. (Seo et al . 40 ) = 500 μ m. Magnification = c, d ×

and physical properties, 38 but use of the silk material alone did not
enablesu cient attachment orgrowth ofcells.
Recently, a reinforced composite silk scaffold was designed and
found to have the mechanical properties of a silk material, while
enablingincreasedadhesionandproliferationofcellsbylyophilized
collagen-hydroxyapatite (HA) substrates. The collagen-hyaluronan
substratesinthereinforcedsilkscaffoldalsoledtoincreasedratesof
cell migration when compared with silk. Moreover, these substrates
induced angiogenesis, which is essential for the initial phase of
repair of a damaged ligament (Figs. 28.2 and 28.3). 39 , 40

Figure 28.3. Histological comparison of the silk scaffold (a, c) and com-
posite silk scaffold (b, d). The cross section was performed in the center of
the silk and composite scaffolds. (a, b: s = silk fiber; B: Blue color = new
synthesis collagen; H: arrow: blood vessels). Masson Trichrome stain: (a, b)
=× 40,scalebar = 200 μ m.CD31stain:(c,d) =× 200,scalebar = 100 μ m.

(Seo et al . 40 ) See also Color Insert.