Biomedical Engineering Reference
In-Depth Information
gelled is not easily infiltrated into the interior pores of scaffold due
to high viscosity. In order to make a collagen/SMCs mixture infil-
trate into the inside of PLCL scaffold, a decompressing device was
used, as shown in Fig. 27.6a. SEM examinations showed that gelled
collagen, which was observed as a fibrous network (Fig. 27.6b),
was incorporated in the inside of the scaffolds under the decom-
pressed condition. Cell adhesion and proliferation rate increased in
collagen/SMC-incorporated tubular PLCL scaffolds compared with
the scaffolds in whichonly SMCs were seeded. From SEM imageand
histological analysis, we further found that SMCs grew in the inside
as well as on the surface of collagen/SMC-incorporated scaffolds
and the cells continued to grow as a monolayer on collagen fibers.
Four weeks after culture, the pores of the inner lumen, inside sur-
face, and outer surface were covered with grown SMCs, and tissue-
like structure was found in collagen/SMC-incorporated constructs
(Fig. 27.7d), whereas the pores of those were kept open in SMC-
seeded constructs (Fig. 27.7a). In 4 -6-diamidino-2-phenylindole
(DAPI) staining, most cells were grown on the surface of scaffolds
in SMC-seeded scaffolds (Fig. 27.7b). On the other hand, cells were
found to be grown evenly in the inside as well as on the inner/outer
surface in collagen/SMC-incorporated scaffolds (Fig. 27.7e). The
content of elastin was examined to evaluate differentiation
Figure 27.7. SEM micrographs (a, d), DAPI staining (b, e), and elastin
staining (c, g) of tissue-engineered grafts cultured for 4 weeks. (a-c)
Unmodified PLCL scaffolds and (d-f) collagen-incorporated PLCL scaffolds.
See also Color Insert.
 
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