Biomedical Engineering Reference
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withthesurroundingtissue.However,thedefectimplantedwiththe
scaffold without the cells showed no evidence of hyaline cartilage
regeneration.
22.6 Leak-Proof Hybrid Scaffolds
When seeded in porous scaffolds, cells do not always stay and
remain in the scaffolds. They might pass through the porous struc-
tureofthescaffoldsandleakfrom theopenperiphery, resultingina
lossofcellsthataffectscelldistributionand,finally,thequalityofthe
engineeredtissue.Topreventcellleakagefromporousscaffoldsand
increase cell-seeding e
ciency, a novel type of hybrid scaffold has
been developed that has the capacity to prevent cell leakage dur-
ing cell seeding. The leak-proof hybrid scaffolds were prepared by
covering all the surfaces of a collagen sponge except the top surface
with a porous structure containing small pores to prevent cell leak-
age during cell seeding (Fig. 22.8).
To test this approach, a collagen sponge was first wrapped with
a nondegradable nylon mesh.
20
The leak-proof collagen sponge
was prepared in a mold by forming a collagen sponge in a poly-
ethylene cylinder of which the bottom and inside walls were cov-
ered with a nylon membrane. SEM observation showed that a
Figure 22.8.
Illustration of a leak-proof hybrid scaffold.
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