HYBRID POROUS SCAFFOLDS OF BIODEGRADABLE SYNTHETIC POLYMERS AND COLLAGEN FOR TISSUE ENGINEERING - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 429

Figure 22.7. SEM photomicrograph of a collagen/PLGA-collagen biphasic
sponge.
other layer was a hybrid sponge with a collagen sponge formed in
the pores of the PLGA sponge. The collagen sponges in the two lay-
ers were connected.
The collagen/PLGA-collagen biphasic hybrid scaffold has
been used for osteochondral tissue engineering. Canine bone
marrow-derived MSCs were isolated from bone marrow aspirate of
a one-year-old beagle and subcultured in DMEM containing 10%
FBS. The subcultured MSCs were seeded into the collagen/PLGA-
collagen biphasic sponge scaffold, cultured in vitro for one week,
and transplanted into the knee of the same beagle. The implants
were harvested after three months. Gross appearance showed that
the defect treated with the cells/scaffold presented a smoother sur-
face and better integration with the surrounding tissue than did a
scaffold without cells. Histological examination of these specimens
using hematoxylin and eosin and safranin O/fast green staining
indicated that cartilage-like and underlying bonelike tissues were
regenerated after four months' implantation. The cartilage-like tis-
sue was stained intensively with safranin O and well integrated