Biomedical Engineering Reference
In-Depth Information
Figure 21.4.
Comparisonofliver-specificfunctionssuchasalbuminsecre-
tion (a), ammonia elimination (b), and EROD activities (c) in AL and
AL/GCspongesformonocultureandcocultureofhepatocyteswithNIH3T3.
(a)Albuminsecretionratesforbothsystemsweremeasuredover28daysin
culture. ELISA specific for mouse albumin was used to quantify protein in a
24-h conditioned medium. (b) Ammonia elimination was compared
between both systems on days 5 and 16 of culture. (c) EROD deethylase
activity by cytochrome P4501A1 was compared between both systems on
days 6 and 16 of culture. EROD activity was measured using the highly
fluorescent resorufin in both systems. All data is represented as mean
±
SD(
n
−
1) (where
n
=
6). Statistics (two-tailed paired Student's
t
-test)
compared monoculture with coculture; *
P
<
0.05 and **
Po
<
0.01. (From
Ref.54).
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