EXTRACELLULAR MATRIX–BASED SCAFFOLDS FROM SCRATCH - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 395

(COL), at 21 days still ∼ 20% of cells were hypoxic. This clearly
demonstrates the important effect growth factors have in acellular
scaffolds intissue engineering.
20.5 Future Outlook
The construction of scaffolds with both a defined morphology and
a defined composition allows the preparation of the ECMs for tis-
sue engineering from scratch. These artificial ECMs can be designed
such that they harbor and provide the correct signals for the cells to
populate the scaffolds and to regenerate the desired tissue, as seen
in our in vivo experiment. When specific effector molecules (growth
factors/morphogens)importantinembryonicdevelopmentofapar-
ticular organ are incorporated in the scaffolds, organogenesis may
even be recapitulated in the adult situation.
Acknowledgments
ResearchdescribedinthischapterwasfundedbytheDutchProgram
forTissueEngineering(DPTE6735andDPTE6739),theFP6-project
EuroSTEC (contract LSHB-CT-2006-037409), ZonMW 920-03-304,
and ZonMW920-03-456.
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