Biomedical Engineering Reference
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Figure 18.5. RT-PCR analysis for Fas, P51, and Bax expression of human
ASCsculturedonplates,trypsinized,andsubsequentlyculturedonmacrop-
orous PLGA microspheres for 1 day (white bars) and human ASCs cultured
on macroporous PLGA microspheres.
10 days. Reverse transcriptase-polymerase chain reaction (RT-PCR)
analysis showed that the ASCs cultured on macroporous PLGA
microspheresexhibitedsignificantlylowermRNAexpressionofFas,
P51, and Bax, which are proapoptotic genes, than those cultured
on plates, trypsinized, and subsequently cultured on macroporous
PLGA microspheres forone day (Fig. 18.5).
18.4 Macroporous PLGA Microsphere as
an ASC Transplantation Vehicle
ASCs were cultured on macroporous PLGA microspheres with a
growth medium for 7 days and then with the adipogenic differen-
tiation medium for 10 days in spinner flasks and implanted into
the subcutaneous space of mice. As a control, ASCs were cultured
with the adipogenic differentiation medium on culture plates for
10 days, trypsinized, mixed with macroporous PLGA microspheres,
and immediately injected into the subcutaneous space of athymic
mice. Six weeks after implantation, ASCs cultured on microspheres
and transplanted with the microspheres without trypsinization
(group 1) had better in vivo adipogenic regenerative e cacy than
ASCs cultured on plates, trypsinized, and subsequently mixed with
 
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