Biomedical Engineering Reference
In-Depth Information
Chapter 18
POROUS POLY(LACTIC-
CO
-GLYCOLIC
SUBSTRATE AND CELL
TRANSPLANTATION VEHICLE
Byung-Soo Kim
Department of Bioengineering, Hanyang University, 17 Haegdang-dong,
Seongdong-gu, Seoul, Korea
bskim@hanyang.ac.kr
Porous poly(lactic-
co
-glycolic acid) (PLGA) microspheres can be
utilized both as a cell culture substrate to expand cells
ex vivo
and as a cell transplantation vehicle for tissue engineering or cell
therapy.
Ex vivo
cell expansion on porous PLGA microspheres and
subsequent transplantation of the cell-microsphere constructs
removes the trypsinization process used to harvest
ex vivo
-
expanded cells for transplantation. This would be advantageous
sincetrypsinizationinterruptsinteractionsbetweenculturedcells
and their extracellular matrices, facilitating apoptosis and conse-
quently limiting the therapeutic e
cacy of the transplanted cells.
Stem cells can be cultured on macroporous PLGA microspheres
in stirred suspension bioreactors, expanded, and differentiated
into a specific lineage. The apoptotic activity of cells cultured on
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