Biomedical Engineering Reference
In-Depth Information
Figure 17.4.
A design of an artificial blood vessel (a) and growth of coro-
naryarteryendothelialcellsonvesselsincubatedwithCBD-HGForHGF(b).
Cells were stained with an anti-CD31antibody.
therightventricularwallsofamechanicallyinjuredporcineheart.
49
An increase in contractility and electrical activity was noticed 60
days after surgery, which was accompanied with angiogenesis and
the appearance ofcardiomyocyte-like cells in the injured region.
17.4.3
Nerve Regeneration
Fibrin-bindingNGF(TG-P-NGF)mixedwithfibringelenhancesneu-
riteextensionfromchickembryodorsalrootganglia(organculture)
by 50% relative to native NGF and by 350% relative to the fibrin gel
alone, in spite of the reduced activity of the fusion construct.
30
Collagen-binding NGF (CBD-NGF)
23
combined with a collagen
membrane and implanted subcutaneously enhances nerve growth
into the membrane; the CBD-NGF-mediated nerve fiber density is
approximately twice that observed for native NGF. It also enhances
healing of rabbitdermal ischemiculcers.
17.4.4
Bone Regeneration
A collagen sponge impregnated with collagen-binding BMP3 (rh-
BMP3-C) failed to induce ectopic bone formation in rats. This may
coincide with a report that denies the osteoinductive activity of
BMP3.
50
However, the authors showed that porous ceramics coated
with collagen and combined with the recombinant protein induce
cranial repair.
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