Biomedical Engineering Reference
In-Depth Information
factors, as reviewed by Ito.
7
,
8
However, one report showed a
very slow release and internalization of a bound growth factor.
46
One of the advantages of binding growth factors is the combina-
tion of natural tissues or artificial scaffolds by simple addition.
Therefore, binding growth factors have been studied regard-
ing their e
cacy in wound healing, tissue reconstruction, and
substitution.
17.4.1
Skin Wound Repair
The wound-healing effect of engineered EGF combined with matrix
components was tested. Collagen-binding EGF (FNCBD-EGF) mixed
with collagen gel stays in the wound, stimulates cell proliferation,
and enhances the formation of granulation tissue in intractable
skin wounds of diabetic mice (db/db).
27
Implantation of a collagen
sponge combined with FNCBD-EGF induces epithelialization above
or underneath the sponge, which results in wound closure.
24
Native
EGF does not exhibit these wound-healing effects. Collagen-binding
bFGF (rhbFGF-F2) dissolved in collagen solution accelerates wound
healing, bothin normal andin diabetic rats.
14
Collagen-binding PDGF-BB combined with a collagen membrane
accelerates the healing of rabbit dermal wounds (ischemic ulcers),
with concomitant capillary formation.
47
Its effect, compared with
nativePDGF-BB,apparentlyimprovesaftertwoweeks(butnotafter
oneweek)ofimplantation.Hall
et al
.
15
showedthatcollagen-binding
EGF (EGF-CBD) instillated into an injured colon without a carrier
promotes the repair ofthe inflamed luminal surface.
Fibrin-binding KGF (P-KGF) in combination with fibrin showed
a potential for wound healing of human skin tissues grafted on
athymic mice,
32
although its e
cacy was not great compared with
that of nativeKGF/fibrin.
17.4.2
Repair of Cardiovascular Tissues
The angiogenic factors VEGF, bFGF, and HGF have been studied for
their therapeutic potential to restore blood supply in ischemic tis-
sues.Theeffectsofgene-engineeredconstructsofthesefactorswere
also studied.
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