Biomedical Engineering Reference
In-Depth Information
Table17.1. Thegene-engineeredbindinggrowthfactorsreportedtodate,manyofwhicharematrix-bindinggrowthfac-
tors.Itiswellknownthatmostgrowthfactorshavea
nitytomatrixproteins,andtheECMisconsideredasthereservoir
of growth factors.
10
,
11
However, this a
nity is not usually strong enough for prolonged retention of the growth factors at
theECM.
12
Therefore, artificial improvement of the matrix-binding a
nity of growth factors hasbeen tried.
Binding target
Growth factor
Fused polypeptide
Origin
Ref.
CBD polypeptide
a
(10 amino acids)
Collagen Gelatin
bFGF, TGF
β
1, BMP3, EGF
vWF
14-17, 21
EGF, bFGF
CBD (20 KDa)
bacterial collagenase
18, 19
CBD polypeptide
b
(eight amino acids)
PDGF-BB, BMP2, bFGF, NGF
humancollagenase
20-23
EGF, VEGF, HGF
CBD (40 or 27 KDa)
Fibronectin
24-26
EGF
Collagen (fullsize)
Collagen
29
FXIIIa substratesequence
c
Fibrin Fibrinogen
VEGF, BMP2, NGF, KGF, ephrin B2
α
2-plasmin inhibitor
30-35
(eight or 12 amino acids)
EGF
FBD (11 Kda)
Fibronectin
37
Cell (integrin)
bFGF, EGF
Cell-binding domain (30 Kda)
Fibronectin
38, 39
EGF
RGD
Fibronectin
40, 41
Cell (integrin and IGF-I R)
IGF-I
vitronectin (full size)
Vitronectin
42
Ti-binding peptide motif
d
Ti
BMP2
artificial
43
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