Biomedical Engineering Reference
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IL - 1
b
TNF -
a
b
- actin
D4
D0
D1
D2
D8
Figure 16.9.
The number of viable HL-60 cells on PLGA and DBP/PLGA
scaffoldsatDays1,2,and5,asdetermined bytheMTTcolorimetric assay. *
corresponds to
p
<
0.05 in comparison with PLGA scaffolds for each day.
Figure 16.10.
TheexpressionofTNF-
mRNAonHL-60cellsfellcontinu-
ously with an increase in the content of DBPs in PLGA. There is a significant
decreaseofTNF-
α
α
expressiononHL-60cellsineachscaffoldasDBPcontent
increases from 0% to80%.
comparedwithPLGAfilms.NosignificantdifferencesofIL-1
mRNA
expressionwereobservedbetweenPLGA,10%DBP/PLGA,and20%
DBP/PLGA (Fig. 16.11).
β
16.3.3 In vivo
Tissue Response
Tofurthercharacterizethe
invivo
inflammatoryresponsesurround-
ing the implants, histological examination was performed at Day
5 after implantation. Remarkable inflammation was observed in
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