SYNTHETIC/NATURAL HYBRID SCAFFOLD FOR TISSUE REGENERATION - Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine - page 325

Figure 16.8. The number of viable HL-60 cells on PLGA and DBP/PLGA
scaffoldsatDays1,2,and5,asdetermined bytheMTTcolorimetric assay. *
corresponds to p < 0.05 in comparison with PLGA scaffolds for each day.

and the 10% DBP/PLGA scaffold at Days 2 and 3. Next we evaluated
the viability of HL-60 cells on samples and did not find statistical
significances between PLGA and DBP hybrid scaffolds at Days 1 and
2.BycultureDay5,proliferationofHL-60cellswithPLGAincreased
slightly more than withDBP/PLGA scaffolds(Fig. 16.8).

16.3.2 Inflammatory Cytokine Expression
Toelucidatethecellularresponsesassociatedwithinflammationon
sample films, we measured the level of mRNA expression of tumor
necrosis factor- α (TNF- α ) and IL-1 β from HL-60 cell in 48 hours
after culture with PLGA or DBP/PLGA films, as shown in Fig. 16.9.
TNF- α mRNA in HL-60 highly expressed following PLGA films com-
pared with DBP/PLGA films; it was significantly lower following
DBP/PLGA
films than PLGA films, with increases in contents of DBPs: 10%,
20%,40%,and80%ofDBPs( p < 0 . 005, p < 0 . 0001, p < 0 . 00005,
and p < 0 . 00005, respectively) (Fig. 16.10). The intensity of TNF- α
expression of PLGA films was significantly 10 times higher or more
thanthatof40%DBP/PLGAfilms.HL-60cellswith80%DBP/PLGA
films rarely expressed TNF- mRNA

expressiondecreasedmarkedlywith40%and80%DBP/PLGAfilms

α

mRNA. Similarly, IL-1

β