Biomedical Engineering Reference
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inflammatory reaction and used histological and molecular analy-
ses to assess how cells and tissue responded to DBP-PLGA hybrid
materials invivo and invitro .Weevaluatedtheeffectoffivedifferent
ratios of DBP/PLGA hybrid materials on the cellular inflammatory
response andtissue reaction induced by PLGA. 6
16.3.1 Cell Viability
In order to evaluate the influence of DBP content in PLGA materi-
als on cells, we analyzed the viability of mouse fibroblasts on PLGA
and the five different ratios of DBP/PLGA scaffolds during the in
vitro culture and found that DBPs enhanced initial attachment of
fibroblasts on the scaffolds. At Day 1, the number of vital cells was
significantly higher in the culture of the scaffold containing DBPs
than that of the scaffold without DBPs (Fig. 16.7). Particularly, 20%
and40%DBP/PLGAscaffoldsmaintainedthenumberofviablecells
comparedwithPLGAscaffoldsthroughthreedays.TherangeofDBP
contents of PLGA scaffolds showed no adverse effects on fibrob-
lastcellattachment,proliferation,andviabilitycomparedwithPLGA
scaffolds. No significant differences were found between the PLGA
Figure 16.7. The number of viable fibroblasts on PLGA and DBP/PLGA
scaffolds at 1, 2, and 3 days as determined by the MTT colorimetric
assay. , § , correspond to p < 0 . 05 in comparison with PLGA scaf-
folds for each day. Abbreviation : MTT, (3-(4,5- Dimethylthiazol -2-yl)-2,5-
di phenyl tetrazolium bromide.
 
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