Biomedical Engineering Reference
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Figure 16.6.
At 2 weeks, cartilaginous tissue formation was more promi-
nent in fibrin/PLGA seeded with NP cells compared with PLGA. Clearly, the
formation of cartilaginous tissue was evident by the third week of
in vitro
culture in fibrin/PLGA. The presence of an accumulated proteoglycan-rich
matrixandGAGatthecoreregionwassignificantandwasintenselystained
at 2 weeks and greatest at 3 weeks. Conversely, NP cells cultured in PLGA
without fibrin demonstrated slower progress of cartilaginous tissue forma-
tion
in vitro
. The differences between the fibrin/PLGA and PLGA groups
were distinguishable in terms of overall cartilaginous tissue formation, cell
organization, and ECMdistribution in all specimens. See also ColorInsert.
distributed in the basophilic ECM in concert with the presence of
specific histochemical property of a proteoglycan-rich matrix and
GAG. Fibrin/PLGA developed a predominantly superior histoarchi-
tecture compared with the PLGA group. In PLGA, a cluster of a few
rounded cells was spotted throughout the specimen. For the NP
specimen, as shown in Fig. 16.6, cartilaginous tissue formation was
also more prominent in fibrin/PLGA than in PLGA.
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