Biomedical Engineering Reference
In-Depth Information
0.30
0.25
0.20
0.15
0.10
0.05
0.00
PHBV film
PHBV fiber
PHBV/gelatin fiber
Figure 13.12.
Cellviabilityformosanabsorbanceexpressed asameasure
of cell viability from NIH3T3 cells cultured onto nanofibrous scaffolds for
5 d. Data is expressed as mean
±
SD (
n
=
5) of the specific absorbance
(adapted from Ref. 17).
a thicker layer was formed, indicating that the composite scaf-
fold allowed for cellular penetration or infiltration inside the
PHBV/gelatin composite fibrous structure.
17
After seven days of incubation, compared with the PHBV film,
the cell viability of fibroblasts cultured on PHBV and PHBV/gelatin
nanofibrous scaffolds was significantly enhanced (
p
<
0
.
01), as
shown in Fig. 13.12.
17
13.6 Conclusions
In this chapter, discussion has been mainly focused on the use
of the electrospinning technique for the immobilization of natural
biopolymers, such as collagen and gelatin, with a synthetic polymer,
such as PHBV, to prepare nanocomposites on the basis of selected
research studies. On the basis of the research results, it has been
demonstratedthatPHBV/proteinsnanocompositesshowhighercell
adhesion, proliferation, and cell viability comparison with those of
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