Biomedical Engineering Reference
In-Depth Information
(a)
(b)
Figure 5.3.
Esterified hyaluronan implanted in (a) humans (16 weeks)
and(b)rats(26weeks);althoughbloodvessels(B)wereobservedinlimited
numbers within the scaffolds, the cells within the scaffolds (S) were mostly
macrophages. Fat cells (FT) were observed only outside the scaffolds and
were part of the subcutaneous adipose layer. See also ColorInsert.
scaffolds. Van Gieson staining demonstrated increased collagen
deposition in seeded scaffolds compared to acellular specimens.
Although seeded implants demonstrated the presence of vascular
structures at the periphery of the scaffolds, few were observed
deeper within the construct. Despite some distinctive differences
between the seeded and acellular groups, no mature adipocytes
were observed withineither group (Fig. 5.3).
A similar effect was observed in pigs
27
and rats.
25
Macrophage
infiltration was observed within the pores of the scaffold, and all
mature adipocytes that were associated with the scaffold were
observed only at the periphery. There were, however, no major dif-
ferences between intramuscular and subcutaneous implantation in
either species.
5.2.2
Strategies for Controlling Inflammation
Hyaluronanisahighlyhydratedhydrogelandisthereforeextremely
hydrophilic. Benzyl esterification of the carboxylic acid moieties
Search WWH ::
Custom Search