True Arrestins and Arrestin-Fold Proteins: A Structure-Based Appraisal - Progress in Molecular Biology and Translational Science - page 37

MSFLGGFFG---PICEIDIVLNDGETRKMAEMKTEDGK-VEKHYLFYDGESVS

VPS26A

. . . . . .

: : : : :: : :

* * * * * * * **

MIEVVAELSRGPVFLAGEALECVVTVTNPLPPTATSASSEALAWASAQIHCQFHASESRV

RGP1

GKVNLAFKQPGKR-------LEHQGIRIEFVGQIELFNDKSNTHEFVNLVKELALPGELT

VPS26A

. . ** . . . ....

: : : : : : :

* * * * * *

ALPPPDSSQPDVQPDSQTVFLPHRGERGQCILSTPPKILFCDLRLDPGESKSYSYSEVLP

RGP1

QSRSYDFEFMQVEKPYESYIGAN------VRLRYFLKVTIVRRLTDLVKEYDLIVHQLAT

VPS26A

.... . . . .

:: : : : : :

* * * ** ** * * * * * *

IEGPPSFRGQSVKYVYKLTIGCQRVNSPITLLRVPLRVLVLTGLQDVRFPQDEAVAPSSP

RGP1

YPDVNNSIKMEVGIEDCLHIEFEYNKSK--YHLKDVIVGKIYFLLVRIKIQHMELQLIKK

VPS26A

. . . . . . .

:::: : :: : :: :

* * ** * * *

RGP1

FLEEDEGGKKDSWLAELAGERLMAATSCRSLHLYNISDGRGKVGTFGIFKSVYRLGEDVV

VPS26A

EITGIGPSTTTETETIAKYEIMD-------------GAPVKGESIPIRLFLAGYDPTPTM

... . . . ..

: : : : : :

** ** * **

RGP1

GTLNLGEGTVACLQFSVSLQTEERVQPEYQRRRGAGGVPSVSHVTHARHQESCLHTTRTS

VPS26A

RDVNKKFSVRYFLNLVLVDEEDRRYFKQQE-----IILWRKAPEKLRKQRTNFHQR----
FSLPIPLSSTPGFCTAIVSLKWEHFEFVTSREPGLVLLPPVEQPEPTTWTGPEQVPVDTF

. . . . . . .

:: ::::: :: :

* * * * * * *

RGP1

VPS26A

--------FESPESQASAEQPEM
SWDLPIKVLPTSPTLASYAAPGPSTSTITI

.

:: :

** *

RGP1

RGP1
Figure 2.6 RGP1 as a new arrestin-fold protein. (A) The amino acid sequences of human
RGP1 and VPS26A were aligned with Clustal Omega. 45 Identity of a given position is
indicated by (*) and homology by either (:) [strong homology] or (.) [weak homology].
The secondary structure of each protein as predicted by PsiPred is indicated above or
below the sequences. The structure of RGP1 was modeled on Phyre 2 in the intensive
mode. It was modeled on four templates, 2FAU (human VPS26A), 1SUJ (Ambystona cone
arrestin), 1AYR (bovine rod arrestin), and 1G4M (bovine b-arrestin 1) with a confidence
value of 99.7-99.9%. (B) The modeled structure of human RGP1 is shown side by
side with that of human VPS26A (2FAU). The characteristic double b-sheet sandwich
is found in both structures and qualifies RGP1 as a novel arrestin-fold protein to be
added to the arrestin clan.

VPS26A