Biomedical Engineering Reference
In-Depth Information
Fig. 2.7
(
a
) The “micro water suspending” technology to examine ice nucleation
.
Illustration of
the sample cell and the placement of a microsized DI water drop. The density of Oil I is smaller
than water, and the density of Oil II is higher than water. Before experiments, both water and
oils were carefully filtrated [
51
]. (
b
) The correlation between ln(£
V
) and 1/
T
(
T
)
2
forDIwater
filtered by a 20 nm filter (
curve A
), 0.5 mg/mL AFPIII solution filtered by a 20 nm filter (
curve B
),
2.5 mg/mL AFPIII solution filtered by a 20 nm filter (
curve C
), and DI water filtered by a 200 nm
filter (
curve D
). Reprinted with permission from ref. [
12
]. Copyright (2004) the American Society
for Biochemistry and Molecular Biology
then be
f
(
m
,
R
0
)
D
,as
f
(
m
,
R
0
)
j
max
D
1). In contrast to this expectation, we obtain
at low T (high 1/(
T
T
2
)) a straight-line segment with the largest slope within the
measurable range of T , whereas at high T (low 1/(
T
T
2
)) we obtain a straight-
line segment with a much smaller slope, the two straight-line segments being joined
by a curve (Fig.
2.7
b). This implies that the nucleation barrier rises abruptly to the
highest level (G
G
homo
,
f
(
m
,
R
0
)
D
1) at low supercoolings as if the foreign