Biomedical Engineering Reference
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Fig. 2.7 ( a ) The “micro water suspending” technology to examine ice nucleation . Illustration of
the sample cell and the placement of a microsized DI water drop. The density of Oil I is smaller
than water, and the density of Oil II is higher than water. Before experiments, both water and
oils were carefully filtrated [ 51 ]. ( b ) The correlation between ln(£ V ) and 1/ T ( T ) 2 forDIwater
filtered by a 20 nm filter ( curve A ), 0.5 mg/mL AFPIII solution filtered by a 20 nm filter ( curve B ),
2.5 mg/mL AFPIII solution filtered by a 20 nm filter ( curve C ), and DI water filtered by a 200 nm
filter ( curve D ). Reprinted with permission from ref. [ 12 ]. Copyright (2004) the American Society
for Biochemistry and Molecular Biology
then be f ( m , R 0 ) D ,as f ( m , R 0 ) j max D 1). In contrast to this expectation, we obtain
at low T (high 1/( T T 2 )) a straight-line segment with the largest slope within the
measurable range of T , whereas at high T (low 1/( T T 2 )) we obtain a straight-
line segment with a much smaller slope, the two straight-line segments being joined
by a curve (Fig. 2.7 b). This implies that the nucleation barrier rises abruptly to the
highest level (G
G homo , f ( m , R 0 ) D 1) at low supercoolings as if the foreign
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