Biomedical Engineering Reference
In-Depth Information
Xe lamp
Aperture
Lens
Field diaphragm
CCD
Lens
Pinhole
Objective
Sample
Beamsplitter Beamsplitter
Spectrometer
Fig. 8.4 Schematic of a home-built microspectrometer at Fudan University that enables spectral
detections of microscopic regions. It is based on an optical microscope with the beam size
controlled by the field diaphragm and pinhole
a
b
Fig. 8.5 ( a ) Schematic of an integrating sphere for reflection measurements. ( b ) Schematic of a
double-integrating sphere for transmission measurements
Transmission measurements require two integrating spheres: one serves as a
uniform light source and the other is used to collect transmitted light. Absorptance
A can also be obtained, from the formula A
D
1 R T ,where R is reflectance
and T is transmittance.
8.4.3
Color Specifications
Any possible color can be specified according to their hue, saturation, and bright-
ness. The hue of a color can be considered as the peak wavelength in its reflection
spectrum. Saturation tells the purity of the color, characterized by the peak width.
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